RESEARCH ARTICLE
MHC Class II-Restricted Presentation of the Major House Dust Mite Allergen Der p 1 Is GILT-Dependent: Implications for Allergic Asthma
Abstract
Gamma-interferon-inducible lysosomal thiol reductase (GILT) is known to reduce disulfide bonds present in proteins internalized by antigen presenting cells, facilitating optimal processing and presentation of peptides on Major Histocompatibility Complex class II molecules, as well as the subsequent activation of CD4-positive T lymphocytes. Here, we show that GILT is required for class II-restricted processing and presentation of immunodominant epitopes from the major house dust mite allergen Der p 1. In the absence of GILT, CD4-positive T cell responses to Der p 1 are significantly reduced, resulting in mitigated allergic airway inflammation in response to Der p 1 and house dust mite extracts in a murine model of asthma.
Citation: West LC, Grotzke JE, Cresswell P (2013) MHC Class II-Restricted Presentation of the Major House Dust Mite Allergen Der p 1 Is GILT-Dependent: Implications for Allergic Asthma. PLoS ONE 8(1): e51343. doi:10.1371/journal.pone.0051343
Editor: Susanne Krauss-Etschmann, Ludwig-Maximilians-University Munich, Germany
Received: July 9, 2012; Accepted: November 5, 2012; Published: January 11, 2013
Copyright: © 2013 West et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This work was supported by the American Asthma Foundation, the Howard Hughes Medical Institute, and the National Institutes of Health Training Grant from T32AI07019, Yale Immunobiology. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
* E-mail: peter.cresswell@yale.edu
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