Int. J. Mol. Sci. 2013, 14(8), 16958-16969; doi:10.3390/ijms140816958
Article
MicroRNA-143 Downregulates Interleukin-13 Receptor Alpha1 in Human Mast Cells
1 Department of Otolaryngology, Huadong Hospital, Fudan University, Shanghai 200040, China2 Department of Otolaryngology, Tongji Hospital, Tongji University, Shanghai 200065, China3 Department of Otolaryngology, Jinan General Hospital of PLA, Jinan 250031, Shandong, China
* Author to whom correspondence should be addressed.
Received: 3 June 2013; in revised form: 8 July 2013 / Accepted: 2 August 2013 / Published: 19 August 2013
(This article belongs to the Special Issue Regulation by non-coding RNAs)
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Abstract: MicroRNA-143 (miR-143) was found to be downregulated in allergic rhinitis, and bioinformatics analysis predicted that IL-13Rα1 was a target gene of miR-143. To understand the molecular mechanisms of miR-143 involved in the pathogenesis of allergic inflammation, recombinant miR-143 plasmid vectors were constructed, and human mast cell-1(HMC-1) cells which play a central role in the allergic response were used for study. The plasmids were transfected into HMC-1 cells using a lentiviral vector. Expression of IL-13Rα1 mRNA was then detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western Blotting. The miR-143 lentiviral vector was successfully stably transfected in HMC-1 cells for target gene expression. Compared to the control, the target gene IL-13Rα1 was less expressed in HMC-1 transfected with miR-143 as determined by RT-PCR and Western Blotting (p < 0.05); this difference in expression was statistically significant and the inhibition efficiency was 71%. It indicates that miR-143 directly targets IL-13Rα1 and suppresses IL-13Rα1 expression in HMC-1 cells. Therefore, miR-143 may be associated with allergic reaction in human mast cells.
Keywords: microRNA-143; IL-13Rα1; mast cell; lentiviral vector; allergy
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