Published online 2013 August 27. | |||
| Copyright © 2013 The Korean Academy of Asthma, Allergy and Clinical Immunology • The Korean Academy of Pediatric Allergy and Respiratory Disease | |||
Open Access
Pernille Munk Frandsen, 1 Inge Jacoba Maria Kortekaas Krohn,2 Hans Jürgen Hoffmann,2 and Peter Oluf Schiøtz1 | |
| 1Pediatric Research Lab, Aarhus University Hospital, Denmark. | |
| 2Clinical Institute & Department of Respiratory Medicine, Aarhus University Hospital, Denmark. | |
| Correspondence to: Pernille Munk Frandsen, MSc, Pediatric Research Lab (A-lab), Aarhus University Hospital, Skejby, Brendstrupgaardsvej 100, DK-8200 Aarhus N, Denmark. Tel: +45-78451520; Fax: +45-87150201; | |
| Received November 14, 2012; Accepted December 26, 2012. | |
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Abstract
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| Purpose
The mast cell plays a pivotal role in the human immune response. Crosslinking of 2 IgE molecules bound to the high affinity IgE receptor (FcεRI) on the surface of the mast cell results in mast cell degranulation and the release of several proinflammatory mediators. Patients with type-I allergy have increased levels of IgE in the blood compared to healthy individuals.
Methods
In a 6-week culture system of stem cells to human mast cells we investigated the effect of the concentration of IgE. The mast cells were cultured with different concentrations of IgE for the last 10 days of the maturation period. It was observed how the IgE concentration affects the histamine release, FcεRI density on the mast cell surface and the concentration of other mediators.
Results
A clear correlation between IgE concentration in culture medium and the release of histamine upon activation was observed. It showed a bell-shaped dose response curve, with maximal response around an IgE-concentration of 250 ng/mL. Furthermore, the sensitivity of the mast cells and surface density of FcεRI on mast cell surface was also influenced by the IgE concentration in the culture medium.
Conclusions
IgE in the culture medium during the last 10 days of mast cell maturation influences the release of the preformed mediator histamine after mast cell activation and the density of FcεRI on the mast cell surface. The release of the de novo synthetized mediator prostaglandin D2 and the expression of chymase and tryptase are not influenced by IgE in culture medium.
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Keywords: Human mast cell, FcεRI, histamine, prostaglandin D2, IgE, allergy, parasite infection, hygiene hypothesis.
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