- PLoS One
- v.8(2); 2013
- PMC3574067
PLoS One. 2013; 8(2): e56830.
Published online 2013 February 15. doi: 10.1371/journal.pone.0056830
PMCID: PMC3574067
Tao Chen,1 Zai-pei Guo,1,* Li Li,1 Meng-meng Li,1 Ting-ting Wang,1 Rui-zhen Jia,2 Na Cao,1 and Jing-yi Li1
Tohru Fukai, Editor
Abstract
Our previous work indicated that TWEAK is associated with various types of cutaneous vasculitis (CV). Herein, we investigate the effects of TWEAK on vascular injury and adhesion molecule expression in CV mice. We showed that TWEAK priming in mice induced a local CV. Furthermore, TWEAK priming also increased the extravasation of FITC-BSA, myeloperoxidase activity and the expression of E-selectin and ICAM-1. Conversely, TWEAK blockade ameliorated the LPS-induced vascular damage, leukocyte infiltrates and adhesion molecules expression in LPS-induced CV. In addition, TWEAK treatment of HDMECs up-regulated E-selectin and ICAM-1 expression at both mRNA and protein levels. TWEAK also enhanced the adhesion of PMNs to HDMECs. Finally, western blot data revealed that TWEAK can induce phosphorylation of p38, JNK and ERK in HDMECs. These data suggest that TWEAK acted as an inducer of E-selectin and ICAM-1 expression in CV mice and HDMECs, may contribute to the development of CV.
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