On-line version ISSN 1414-431X
Braz J Med Biol Res vol.45 no.8 Ribeirão Preto Aug. 2012 Epub May 17, 2012
http://dx.doi.org/10.1590/S0100-879X2012007500077
Yubao Cui, Ying Zhou, Guifang Ma, Li Yang, Yungang Wang and Weihong Shi
Department of Laboratory Medicine, Yancheng Health Vocational and Technical College, Jiangsu Yancheng, China
Abstract Introduction Material and Methods Results Discussion References Acknowledgments Correspondence and Footnotes
Crude extracts of house dust mites are used clinically for diagnosis and immunotherapy of allergic diseases, including bronchial asthma, perennial rhinitis, and atopic dermatitis. However, crude extracts are complexes with non-allergenic antigens and lack effective concentrations of important allergens, resulting in several side effects. Dermatophagoides farinae (Hughes; Acari: Pyroglyphidae) is one of the predominant sources of dust mite allergens, which has more than 30 groups of allergen. The cDNA coding for the group 5 allergen of D. farinae from China was cloned, sequenced and expressed. According to alignment using the VECTOR NTI 9.0 software, there were eight mismatched nucleotides in five cDNA clones resulting in seven incompatible amino acid residues, suggesting that the Der f 5 allergen might have sequence polymorphism. Bioinformatics analysis revealed that the matured Der f 5 allergen has a molecular mass of 13604.03 Da, a theoretical pI of 5.43 and is probably hydrophobic and cytoplasmic. Similarities in amino acid sequences between Der f 5 and allergens of other domestic mite species, viz. Der p 5, Blo t 5, Sui m 5, and Lep d 5, were 79, 48, 53, and 37%, respectively. Phylogenetic analysis indicated that Der f 5 and Der p 5 clustered together. Blo t 5 and Ale o 5 also clustered together, although Blomia tropicalis and Aleuroglyphus ovatus belong to different mite families, viz. Echimyopodidae and Acaridae, respectively.
Key words: Dermatophagoides farinae (Hughes); Der f 5; Cloning; Expression; Bioinformatics
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