Exon skipping of FcεRIβ eliminates expression of the high-affinity IgE receptor in mast cells with therapeutic potential for allergy

Published online before printNovember 21, 2016, doi:10.1073/pnas.1608520113 PNAS November 21, 2016

1. Glenn Crusea,b,1, 
2. Yuzhi Yinb, 
3. Tomoki Fukuyamaa, 
4. Avanti Desaib, 
5. Greer K. Arthura, 
6. Wolfgang Bäumera,
7. Michael A. Beavenc, and 
8. Dean D. Metcalfeb

Author Affiliations

1. Edited by K. Frank Austen, Brigham and Women's Hospital, Boston, MA, and approved October 26, 2016 (received for review May 27, 2016)

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Significance

We identified an innovative use for the technique of antisense oligonucleotide-mediated exon skipping to specifically target and down-regulate IgE receptor expression in mast cells. Exon skipping is typically used as part of personalized medicine, where a mutant exon is skipped after sequencing the patients’ affected genes. Our approach, however, targets a nonmutated gene and an exon that is critical for surface IgE receptor expression. It does not require a personalized approach with genetic sequencing or multiple iterations of oligonucleotides that would require clinical trials. Furthermore, the diseases to be treated with this technology are ideal for local delivery of the oligonucleotides by aerosols or topical cream formulations.

Dose-dependence of protection from systemic reactions to venom immunotherapy by omalizumab

Clinical and Molecular Allergy

• Elisa BoniEmail authorView ORCID ID profile,
• Cristoforo Incorvaia and
• Marina Mauro

Clinical and Molecular Allergy201614:14

DOI: 10.1186/s12948-016-0051-2

Abstract

Background

Systemic reactions (SR) to venom immunotherapy (VIT) are rare but may occur, with a rate significantly higher for honeybee than for vespid VIT. In patients with repeated SRs to VIT it is difficult to reach the maintenance dose of venom and pre-treatment with omalizumab is indicated, as shown by some studies reporting its preventative capacity, when antihistamines and corticosteroids are ineffective.

Inappropriate asthma therapy—a tale of two countries: a parallel population-based cohort study

• npj Primary Care Respiratory Medicine 26, Article number: 16076 (2016)
• doi:10.1038/npjpcrm.2016.76
• Article | OPEN

Manon Belhassen

, Anjan Nibber

, Eric Van Ganse

, Dermot Ryan

, Carole Langlois

, Francis Appiagyei

,Derek Skinner

, Laurent Laforest

, Joan B Soriano

 & David Price

Abstract: Against recurrent controversies around the safety of short- and long-acting β2-agonists (SABA and LABA), and the National Review of Asthma Deaths inquiry in the United Kingdom, we investigated the prevalence of inappropriate therapy in asthma. Our study aimed to determine the prevalence of inappropriate use of asthma therapy in the United Kingdom and in France.

Diagnosis of exercise-induced anaphylaxis: current insights

J Asthma Allergy. 2016; 9: 191–198.

Published online 2016 Oct 27. doi:  10.2147/JAA.S109105

Abstract

Exercise-induced anaphylaxis (EIAn) is defined as the occurrence of anaphylactic symptoms (skin, respiratory, gastrointestinal, and cardiovascular symptoms) after physical activity. In about a third of cases, cofactors, such as food intake, temperature (warm or cold), and drugs (especially nonsteroidal anti-inflammatory drugs) can be identified. When the associated cofactor is food ingestion, the correct diagnosis is food-dependent EIAn (FDEIAn). The literature describes numerous reports of FDEIAn after intake of very different foods, from vegetables and nuts to meats and seafood.

Diagnosis and classification of idiopathic inflammatory myopathies

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1. Previous article in issue: The juvenile idiopathic inflammatory myopathies: pathogenesis, clinical and autoantibody phenotypes, and outcomes
2. Next article in issue: Diagnostic criteria for inclusion body myositis

View issue TOC
Volume 280, Issue 1
July 2016
Pages 39–51

Review

Authors

• First published: 19 June 2016Full publication history
• DOI: 10.1111/joim.12524View/save citation
• 
  

• Abstract
• The idiopathic inflammatory myopathies (IIMs) are a heterogeneous group of diseases, collectively termed myositis, sharing symptoms of muscle weakness, fatigue and inflammation. Other organs are frequently involved, supporting the notion that these are systemic inflammatory diseases. The IIMs can be subgrouped into dermatomyositis, polymyositis and inclusion body myositis. The myositis-specific autoantibodies (MSAs) identify other and often more distinct clinical phenotypes, such as the antisynthetase syndrome with antisynthetase autoantibodies and frequent interstitial lung disease and anti-SRP and anti-HMGCR autoantibodies that identify necrotizing myopathy.

Prospective evaluation of testing with baked milk to predict safe ingestion of baked milk in unheated milk-allergic children

• • 
    

• Allergy, Asthma & Clinical Immunology

• Email author
  View ORCID ID profile

Allison Kwan, Maria Asper, Sasson Lavi, Elana Lavine, David Hummel and Julia E. UptonAllergy, Asthma & Clinical Immunology201612:54 DOI: 10.1186/s13223-016-0162-9

Abstract

Background

Cow’s milk allergy is one of the most common food allergies affecting young children. A subset of milk-allergic individuals can eat baked milk without allergic symptoms which is beneficial in terms of prognostication and liberalization of the diet. A retrospective study suggested that skin prick testing (SPT) with a baked milk (muffin) slurry may provide a sensitive means of predicting the outcome of a medically supervised baked milk oral food challenge. We evaluated the predictive value of SPT with baked milk to identify unheated milk-allergic children who are able to safely eat baked milk.

The Emperor Has No Symptoms: The Risks of a Blanket Approach to Using Epinephrine Autoinjectors for All Allergic Reactions

November–December, 2016Volume 4, Issue 6, Pages 1143–1146

Paul J. Turner, FRACP, PhDPress enter key for correspondence informationPress enter key to Email the author

, 

Audrey DunnGalvin, PhD

, 

Jonathan O'B. Hourihane, MD, DM

Open Access

DOI: http://dx.doi.org/10.1016/j.jaip.2016.05.005

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Article Info

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Article Outline

1. Can Using Epinephrine Autoinjectors for Mild Allergic Symptoms Cause Harm?
2. Conclusions
3. References

Fatal anaphylaxis in humans is rare and unpredictable. We note a trend to provide allergic individuals with care plans that recommend immediate use of epinephrine autoinjectors if allergen ingestion is suspected, even in the absence of any allergic symptoms, without any supporting evidence base. Instructions to use an autoinjector device, irrespective of reaction severity and especially when symptoms are actually absent, are likely to add to parental and patient anxiety.

A multi-strain Synbiotic may reduce viral respiratory infections in asthmatic children: a randomized controlled trial

• Electron Physician
• v.8(9); 2016 Sep
• PMC5074739

Electron Physician. 2016 Sep; 8(9): 2833–2839.

Published online 2016 Sep 20. doi:  10.19082/2833

PMCID: PMC5074739

Hamid Ahanchian,1,2 Seyed Ali Jafari,3 Elham Ansari,4 Toktam Ganji,5 Mohammad Ali Kiani,3 Maryam Khalesi,5 Tooba Momen,6 and Hamidreza Kianifar3

Author information ► Article notes ► Copyright and License information ►

Abstract

Background and objective

Asthma is a growing problem worldwide. Acute exacerbations impose considerable morbidity, mortality, and increased cost. Viral respiratory infections are the most common cause (80–85%) of pediatric asthma exacerbations and admissions to the hospital. The aim of this study was to determine the effect of a new synbiotic Lactocare® on viral respiratory infections and asthma exacerbations in asthmatic children.