May 23, 2013

Avidity Studies in Anisakis simplex-Associated Allergic Diseases


Journal of Allergy
Volume 2013 (2013), Article ID 106781, 6 pages
http://dx.doi.org/10.1155/2013/106781
Clinical Study

Avidity Studies in Anisakis simplex-Associated Allergic Diseases

1Departamento de Parasitología, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, Spain
2Servicio de Alergia, Instituto de Investigación Sanitaria, Hospital Universitario de la Princesa, C/Diego de León 62, 28006 Madrid, Spain
Received 8 March 2013; Revised 29 April 2013; Accepted 7 May 2013
Academic Editor: S. L. Johnston
Copyright © 2013 Carmen Cuéllar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Gastroallergic anisakiasis (GAA) and Anisakis-sensitization-associated chronic urticaria (CU+) differ with respect to specific IgE levels. We hypothesised different immunoglobulin avidities in both entities as well as their dependence on TI and fish consumption. 16 patients with GAA and 17 patients with CU+ were included, and immunoglobulin levels were analysed by CAP (Phadia). IgE and IgG avidity indexes (AvIgE and AvIgG, resp.) were also determined. IgG avidity was higher in GAA than in CU+ (), whereas there was a tendency to lower IgE avidity in GAA (). When analysing all patients, AvIgG was positively correlated with specific IgE, IgG, and IgG4 as well as total IgE (Rho between 0.66 and 0.71); but AvIgE was negatively correlated with specific IgE (Rho −0.57) , specific IgG4 (Rho −0.38) , and total IgE (Rho 0.66). In GAA, weekly fish consumption was positively associated with AvIgE (Rho 0.51; ). A multivariate regression showed that time interval was the main explaining factor for AvIgE in GAA. We could show a differential behaviour of immunoglobulin isotype avidities in both entities and their dependence on fish-eating habits as well as on the time elapsed to the last parasitic episode.

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