PLoS One. 2013; 8(6): e66644.
Elevation of Eosinophil-Derived Neurotoxin in Plasma of the Subjects with Aspirin-Exacerbated Respiratory Disease: A Possible Peripheral Blood Protein Biomarker
Helene F. Rosenberg, Editor
Abstract
Aspirin-exacerbated respiratory disease (AERD) remains widely underdiagnosed in asthmatics, primarily due to insufficient awareness of the relationship between aspirin ingestion and asthma exacerbation. The identification of aspirin hypersensitivity is therefore essential to avoid serious aspirin complications. The goal of the study was to develop plasma biomarkers to predict AERD. We identified differentially expressed genes in peripheral blood mononuclear cells (PBMC) between subjects with AERD and those with aspirin-tolerant asthma (ATA). The genes were matched with the secreted protein database (
http://spd.cbi.pku.edu.cn/) to select candidate proteins in the plasma. Plasma levels of the candidate proteins were then measured in AERD (n
=
40) and ATA (n
=
40) subjects using an enzyme-linked immunosorbent assay (ELISA). Target genes were validated as AERD biomarkers using an ROC curve analysis. From 175 differentially expressed genes (
p-value <0 .0001="" 11="" 12="" 4="" 7="" aerd="" among="" and="" as="" ata="" compared="" database="" decreased="" elevated="" em="" eosinophil-derived="" expression="" for="" g="" gene="" genes="" higher="" in="" levels="" ml="" neurotoxin="" observed="" of="" patterns="" plasma="" predicted="" protein="" proteins="" queried="" retrieved.="" secreted="" significantly="" subjects.="" subjects="" that="" the="" these="" to="" vs.="" were="" with="">p
-value <0 .0003="" 0.74="" analysis="" auc="" based="" curve="" em="" on="" roc="" the="" was="">p-value
=
0.0001, asymptotic 95% confidence interval [lower bound: 0.62, upper bound: 0.83]) with 95% sensitivity, 60% specificity, and a cut-off value of 27.15 µg/ml. Eosinophil-derived neurotoxin represents a novel biomarker to distinguish AERD from ATA.
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