- Ann Dermatol
- v.25(3); Aug 2013
- PMC3756195
Ann Dermatol. 2013 August; 25(3): 310–314.
Published online 2013 August 13. doi: 10.5021/ad.2013.25.3.310
PMCID: PMC3756195
Abstract
Background
Malassezia species (spp.) are cutaneous opportunistic pathogens and associated with various dermatological diseases including seborrheic dermatitis, dandruff and atopic dermatitis. Almost allMalassezia spp. are obligatorily lipid-dependent, which might be caused by lack of the myristic acid synthesis. Recent genome analysis of M. restricta and M. globosa suggested that the absence of a gene encoding fatty acid synthesis might be compensated by abundant genes encoding hydrolases, which produce fatty acids, and that lipases and phospholipases may play a role in virulence of the fungus.
Objective
The current study aimed to investigate the contribution of lipases and phospholipases in virulence of theM. restricta as being the most frequently isolated Malassezia spp. from the human skin.
Methods
Swap samples of two different body sites of at least 18 patients with seborrheic dermatitis were obtained and in vivo expression of lipases and phospholipases of M. restricta was analyzed by the gene specific two-step nested RT-PCR.
Results
The results of the current study suggest that majority of the patients display expression of lipase RES_0242.
Conclusion
These data imply a possible role of lipase in the host environment to produce free fatty acids for the fungus.
Keywords: Lipase, Malassezia, Nested reverse transcriptase PCR, Phospholipases, Seborrheic dermatitis
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