Histamine downregulates the Th1-associated chemokine IP-10 in monocytes and myeloid dendritic cells

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Vol. 163, No. 1, 2014

Issue release date: January 2014

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Int Arch Allergy Immunol 2014;163:11-19 
(DOI:10.1159/000355960)

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Original Paper

Glatzer F.a · Mommert S.a · Köther B.a · Gschwandtner M.a, d · Stark H.b, c ·Werfel T.a · Gutzmer R.a  aDivision of Immunodermatology and Allergy Research, Department of Dermatology and Allergy, Hannover Medical School, Hannover, bZAFES/CMP/ICNF, Biocenter, Institute of Pharmaceutical Chemistry, Johann Wolfgang Goethe University, Frankfurt am Main, and cInstitute of Pharmaceutical and Medicinal Chemistry, Heinrich Heine University, Düsseldorf, Germany, dResearch Division of Biology and Pathobiology of the Skin, Department of Dermatology, Medical University of Vienna, Vienna, Austria   Corresponding Author

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  Abstract

Background: Histamine is an important mediator of allergic diseases. It modulates the cytokine expression of various subtypes of antigen-presenting cells by four known receptors, H1R-H4R. The effects of histamine on myeloid dendritic cells (mDC) are unclear.Methods: Monocytes and mDC were isolated from human PBMC. Histamine receptor expression was evaluated by real-time PCR. Cells were stimulated with histamine and histamine receptor ligands, and restimulated with polyinosinic-polycytidylic acid (poly I:C), and supernatants were analyzed by protein array and ELISA. Results: Monocytes and mDC express H1R and H2R without significant differences between the two cell types, whereas H4R mRNA was significantly higher in mDC compared with monocytes and H3R mRNA was not detected in any cell type. Prestimulation with histamine caused a significant decrease in poly I:C-induced expression of interferon-γ-induced protein (IP-10) in mDC and monocytes. Stimulation with specific H1R, H2R and H4R agonists and antagonists showed that the observed effect was mediated via H2R and H4R in monocytes and mDC.Conclusion: Monocytes and mDC have similar histamine receptor repertoires with regard to H1R, H2R and H3R, but H4R expression is higher on mDC. Histamine stimulation shows similar functional effects on both cell types, i.e., downregulation of TLR3-induced IP-10 production. This might be a new mechanism how histamine fosters a Th2 milieu.

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