- PLoS One
- v.8(5); 2013
- PMC3661689
PLoS One. 2013; 8(5): e63775.
Published online May 22, 2013. doi: 10.1371/journal.pone.0063775
PMCID: PMC3661689
Peripheral Blood MDSCs, IL-10 and IL-12 in Children with Asthma and Their Importance in Asthma Development
Emma H. Wilson, Editor
Abstract
Objective
To investigate myeloid-derived suppressor cell (MDSC) accumulation and interleukin 10 (IL-10) and interleukin 12 (IL-12) levels during the onset of asthma in both pediatric patients and mouse models, as well as their possible roles in the development of asthma.
Methods
Peripheral blood samples were gathered from children with asthma attacks (attack group) and alleviated asthma (alleviated group), as well as two control groups, children with pneumonia and healthy children. The pathological characteristics of asthma in asthmatic mice, budesonide-treated asthmatic mice, and normal control mice were also evaluated by immunohistochemistry (IHC) and hematoxylin and eosin (H&E) staining.
Results
MDSC accumulation and serum IL-10 levels were significantly elevated in the children with asthma compared with the budesonide-treated alleviated group, normal healthy controls, and pneumonia controls (p<0 .05="" differences="" groups="" in="" latter="" no="" p="" showed="" statistical="" the="" those="" three="" whereas="">0.05). The level of serum IL-12 in the asthmatic children was drastically reduced compared to the budesonide-treated alleviated group, healthy controls, and pneumonia controls (p<0 .05="" after="" and="" asthma="" asthmatic="" both="" budesonide="" children="" control="" correlated="" differences="" expression="" groups="" higher="" il-10="" il-12.="" il-12="" in="" increased="" latter="" level="" levels.="" levels="" lower="" mdscs="" mice="" negatively="" no="" normal="" of="" p="" percentage="" positively="" reduced="" serum="" showed="" significant="" significantly="" than="" the="" their="" those="" three="" treatment="" upon="" was="" were="" whereas="" with="">
Conclusion
During the onset of asthma, the accumulation of MDSCs and the level of serum IL-10 increase, while the level of IL-12 decreases. These fluctuations may play an important role in the development of asthma.
No comments:
Post a Comment