Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma

Sonja Koch, PhD ∗

, 

Anna Graser, PhD ∗

, 

Hooman Mirzakhani, MD, PhD, MMSc ∗

, 

Theodor Zimmermann, MD

, 

Volker O. Melichar, MD

, 

Marco Wölfel, MD

, 

Damien C. Croteau-Chonka, PhD

, 

Benjamin A. Raby, MD, MPH

, 

Scott T. Weiss, MD, MS ‡

, 

Susetta Finotto, PhD ‡,Press enter key to Email the author

∗These authors share first authorship.

‡These authors share senior authorship.

Open Access

DOI: http://dx.doi.org/10.1016/j.jaci.2015.11.047

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Article Outline

1. Methods
   1. Participants (PreDicta study)
   2. Isolation and culture of human PBMCs (PreDicta study)
   3. RNA isolation from whole blood (PreDicta study)
   4. Isolation of human CD4+ T cells (Asthma BRIGDE study)
   5. NFATc1 and IRF4 microarray analysis of human peripheral blood CD4+ T cells or whole blood cells (Asthma BRIDGE study)
   6. Adult asthmatic patients with positive skin test results (Asthma BIRDGE study)
   7. Mice
   8. Allergen sensitization and challenge
   9. Isolation of murine lung CD4+ T cells
   10. Isolation and differentiation of murine bone marrow–derived mast cells
   11. Histamine release by murine bone marrow–derived mast cells
   12. Flow cytometric analysis
   13. ELISA
   14. RNA isolation and quantitative real time-PCR
   15. Statistical analysis
2. Results and Discussion
3. References