Cell-derived microparticles and the lung

Dario Nieri, Tommaso Neri, Silvia Petrini, Barbara Vagaggini, Pierluigi Paggiaro, Alessandro Celi

European Respiratory Review 2016 25: 266-277; DOI: 10.1183/16000617.0009-2016

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Abstract

Cell-derived microparticles are small (0.1–1 μm) vesicles shed by most eukaryotic cells upon activation or during apoptosis. Microparticles carry on their surface, and enclose within their cytoplasm, molecules derived from the parental cell, including proteins, DNA, RNA, microRNA and phospholipids. Microparticles are now considered functional units that represent a disseminated storage pool of bioactive effectors and participate both in the maintenance of homeostasis and in the pathogenesis of diseases.

Omalizumab's Impact on Total and Allergen-Specific IgE Levels: A Polyclonal Story

Free Access

Int Arch Allergy Immunol 2016;169:69-70
(DOI:10.1159/000444998)

Eckl-Dorna J.  Department of Otorhinolaryngology, General Hospital Vienna, Medical University of Vienna, Vienna, Austria  Corresponding Author

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Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma

Sonja Koch, PhD ∗

, 

Anna Graser, PhD ∗

, 

Hooman Mirzakhani, MD, PhD, MMSc ∗

, 

Theodor Zimmermann, MD

, 

Volker O. Melichar, MD

, 

Marco Wölfel, MD

, 

Damien C. Croteau-Chonka, PhD

, 

Benjamin A. Raby, MD, MPH

, 

Scott T. Weiss, MD, MS ‡

, 

Susetta Finotto, PhD ‡,Press enter key to Email the author

∗These authors share first authorship.

‡These authors share senior authorship.

Open Access

DOI: http://dx.doi.org/10.1016/j.jaci.2015.11.047

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Article Outline

1. Methods
   1. Participants (PreDicta study)
   2. Isolation and culture of human PBMCs (PreDicta study)
   3. RNA isolation from whole blood (PreDicta study)
   4. Isolation of human CD4+ T cells (Asthma BRIGDE study)
   5. NFATc1 and IRF4 microarray analysis of human peripheral blood CD4+ T cells or whole blood cells (Asthma BRIDGE study)
   6. Adult asthmatic patients with positive skin test results (Asthma BIRDGE study)
   7. Mice
   8. Allergen sensitization and challenge
   9. Isolation of murine lung CD4+ T cells
   10. Isolation and differentiation of murine bone marrow–derived mast cells
   11. Histamine release by murine bone marrow–derived mast cells
   12. Flow cytometric analysis
   13. ELISA
   14. RNA isolation and quantitative real time-PCR
   15. Statistical analysis
2. Results and Discussion
3. References

Respiratory Medication Adherence: Toward a Common Language and a Shared Vision

September–October, 2016Volume 4, Issue 5, Pages 799–801

Eric Van Ganse, MD, PhD, FRCPPress enter key for correspondence informationPress enter key to Email the author

, 

David Price, FRCGP

Open Access

DOI: http://dx.doi.org/10.1016/j.jaip.2016.04.006

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Full TextReferencesThe World Health Organization highlights the importance of optimizing chronic respiratory disease (CRD) medication adherence with a view to improving clinical outcomes and alleviating ever-increasing pressures on the world's health care resources.1, 2 The Global Initiative for Asthma also advocates for optimized adherence, recommending that asthma symptoms and risk be optimized on the lowest dose of therapy appropriate and that high-cost add-on therapies only be considered in patients with severe disease who have persistent symptoms and/or exacerbations despite optimized treatment with high-dose controller medications and treatment of modifiable risk factors.2 

Climate Change and Future Pollen Allergy in Europe

Environ Health Perspect; DOI:10.1289/EHP173

Iain R. Lake,1 Natalia R. Jones,1 Maureen Agnew,1 Clare M. Goodess,1 Filippo Giorgi,2 Lynda Hamaoui-Laguel,3,4 Mikhail A. Semenov,5 Fabien Solomon,2 Jonathan Storkey,5 Robert Vautard,3,4 and Michelle M. Epstein6

open

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• Background: Globally pollen allergy is a major public health problem, but a fundamental unknown is the likely impact of climate change. To our knowledge, this is the first study to quantify the consequences of climate change upon pollen allergy in humans.
  Objectives: To produce quantitative estimates of the potential impact of climate change upon pollen allergy in humans, focusing upon common ragweed (Ambrosia artemisiifolia) in Europe.

Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells

PLoS One. 2016; 11(7): e0158643.

Published online 2016 Jul 29. doi:  10.1371/journal.pone.0158643

Ayako Aoki-Yoshida,1,2 Kiyoshi Yamada,1 Satoshi Hachimura,3 Toshihiro Sashihara,4 Shuji Ikegami,4 Makoto Shimizu,1 and Mamoru Totsuka1,*

Hossam M Ashour, Editor

Author information ► Article notes ► Copyright and License information ►

Abstract

Food allergy is a serious problem for infants and young children. Induction of antigen-specific oral tolerance is one therapeutic strategy. Enhancement of oral tolerance induction by diet is a promising strategy to prevent food allergy in infants. Thus, in this study, we evaluate the effect of probioticLactobacillus gasseri OLL2809 (LG2809) on oral tolerance induction in a mouse model.

Characterizing the inflammatory response in esophageal mucosal biopsies in children with eosinophilic esophagitis

Clinical & Translational Immunology (2016) 5, e88; doi:10.1038/cti.2016.30

OPEN

Wael N Sayej¹, Antoine Ménoret², Anu S Maharjan^2,3, Marina Fernandez¹, Zhu Wang⁴, Fabiola Balarezo⁵, Jeffrey S Hyams¹, Francisco A Sylvester^1,2,3,6 and Anthony T Vella^2,6

1. ¹Department of Pediatrics, Division of Digestive Diseases, Hepatology & Nutrition, Connecticut Children’s Medical Center, Hartford, CT, USA
2. ²Department of Immunology, University of Connecticut Health Center, Farmington, CT, USA
3. ³Department of Pediatrics, Division of Gastroenterology, University of North Carolina School of Medicine, Chapel Hill, NC, USA
4. ⁴Department of Research, Connecticut Children’s Medical Center, Hartford, CT, USA
5. ⁵Department of Pathology and Laboratory Medicine, Hartford Hospital, Hartford, CT, USA

Correspondence: Dr WN Sayej, Department of Pediatrics, Division of Digestive Diseases, Hepatology and Nutrition, Connecticut Children’s Medical Center, 282 Washington Street, Hartford, CT 06106, USA. E-mail:wsayej@connecticutchildrens.org

⁶Co-senior authors.

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Abstract

Eosinophilic esophagitis (EoE) is an emerging allergic, IgE- and non-IgE (Th2 cell)-mediated disease. There are major gaps in the understanding of the basic mechanisms that drive the persistence of EoE. We investigated whether esophageal biopsies from children with EoE demonstrate an inflammatory response that is distinct from normal controls. We prospectively enrolled 84 patients, of whom 77 were included in our analysis, aged 4–17 years (12.8±3.8 years; 81% males). Five esophageal biopsies were collected from each patient at the time of endoscopy.

The epidemiologic characteristics of healthcare provider-diagnosed eczema, asthma, allergic rhinitis, and food allergy in children: a retrospective cohort study

• David A. Hill†Email author,
• Robert W. Grundmeier†,
• Gita Ram and
• Jonathan M. Spergel

†Contributed equally

BMC PediatricsBMC series – open, inclusive and trusted201616:133

DOI: 10.1186/s12887-016-0673-z

©  The Author(s). 2016

Open Peer Review reports

Abstract

Background

The rates of childhood allergic conditions are changing, prompting the need for continued surveillance. Examination of healthcare provider-based diagnosis data is an important and lacking methodology needed to complement existing studies that rely on participant reporting.

Methods

Utilizing our care network of 1,050,061 urban and sub-urban children, we defined two retrospective cohorts: (1) a closed birth cohort of 29,662 children and (2) a cross-sectional cohort of 333,200 children. These cohorts were utilized to determine the epidemiologic characteristics of the conditions studied. Logistic regression was utilized to determine the extent to which food allergy was associated with respiratory allergy.