Journal of Allergy Volume 2013 (2013), Article ID 349520, 13 pages http://dx.doi.org/10.1155/2013/349520
Research Article
Impact of Adiponectin Overexpression on Allergic Airways Responses in Mice
1Department of Environmental Health, Harvard School of Public Health, 665 Huntington Avenue, Boston, MA 02115, USA
2Departments of Physiology and Internal Medicine, University of Manitoba, Winnipeg, MB, R3A 1R9, Canada
3Children’s Hospital, Boston, MA 02115, USA
2Departments of Physiology and Internal Medicine, University of Manitoba, Winnipeg, MB, R3A 1R9, Canada
3Children’s Hospital, Boston, MA 02115, USA
Received 21 March 2013; Accepted 7 May 2013
Academic Editor: Akshay Sood
Copyright © 2013 Norah G. Verbout et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Obesity is an important risk factor for asthma. Obese individuals have decreased circulating adiponectin, an adipose-derived hormone with anti-inflammatory properties. We hypothesized that transgenic overexpression of adiponectin would attenuate allergic airways inflammation and mucous hyperplasia in mice. To test this hypothesis, we used mice overexpressing adiponectin (Adipo Tg). Adipo Tg mice had marked increases in both serum adiponectin and bronchoalveolar lavage (BAL) fluid adiponectin. Both acute and chronic ovalbumin (OVA) sensitization and challenge protocols were used. In both protocols, OVA-induced increases in total BAL cells were attenuated in Adipo Tg versus WT mice. In the acute protocol, OVA-induced increases in several IL-13 dependent genes were attenuated in Adipo Tg versus WT mice, even though IL-13 per se was not affected. With chronic exposure, though OVA-induced increases in goblet cells numbers per millimeter of basement membrane were greater in Adipo Tg versus WT mice, mRNA abundance of mucous genes in lungs was not different. Also, adiponectin overexpression did not induce M2 polarization in alveolar macrophages. Our results indicate that adiponectin protects against allergen-induced inflammatory cell recruitment to the airspaces, but not development of goblet cell hyperplasia.
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