December 26, 2013

Staphylococcal enterotoxin B influences the DNA methylation pattern in nasal polyp tissue: a preliminary study


Letter to the editor

Open Access

Claudina A Pérez-Novo1*†, Yuan Zhang23†, Simon Denil4, Geert Trooskens4, Tim De Meyer4, Wim Van Criekinge4, Paul Van Cauwenberge1, Luo Zhang2 and Claus Bachert15
Author Affiliations
1Upper Airways Research Laboratory, Department of Otorhinolaryngology, Ghent University Hospital, De Pintelaan 185, Ghent B-9000, Belgium
2Department of Otolaryngology, Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, PR China
3Key Laboratory of Otolaryngology, Head and Neck Surgery (Ministry of Education of China), Beijing Institute of Otorhinolaryngology, Beijing 100005, PR China
4Department of Mathematical Modelling, Statistics and Bioinformatics, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium
5Karolinska Institutet, Division of ENT Diseases, CLINTEC, Stockholm, Sweden
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Allergy, Asthma & Clinical Immunology 2013, 9:48  doi:10.1186/1710-1492-9-48

The electronic version of this article is the complete one and can be found online at:http://www.aacijournal.com/content/9/1/48

Received:20 September 2013
Accepted:9 December 2013
Published:16 December 2013
© 2013 Pérez-Novo et al.; licensee BioMed Central Ltd. 
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Abstract

Staphylococcal enterotoxins may influence the pro-inflammatory pattern of chronic sinus diseases via epigenetic events. This work intended to investigate the potential of staphylococcal enterotoxin B (SEB) to induce changes in the DNA methylation pattern. Nasal polyp tissue explants were cultured in the presence and absence of SEB; genomic DNA was then isolated and used for whole genome methylation analysis. Results showed that SEB stimulation altered the methylation pattern of gene regions when compared with non stimulated tissue. Data enrichment analysis highlighted two genes: the IKBKB and STAT-5B, both playing a crucial role in T- cell maturation/activation and immune response.
Keywords: 
Staphylococcus aureus enterotoxin B; Chronic rhinosinusitis and nasal polyps; DNA methylation; MBD2; Whole genome methylation analysis; Hypermethylation

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